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Protein Concentration (A₂₈₀)
Absorbance (A) = εcl
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A₂₈₀ Reading:
Path Length (cm):
i
For standard 1 cm cuvettes, enter 1. For NanoDrop™, the A₂₈₀ is usually normalized to a 1 cm pathlength, so also enter 1.
Extinction Coeff. (ε) Type:
Mass-based (e.g., (mg/mL)⁻¹cm⁻¹)
Molar-based (e.g., M⁻¹cm⁻¹)
Extinction Coeff. (ε) Value:
(mg/mL)⁻¹cm⁻¹
Molecular Weight (MW):
kDa
g/mol
Calculate
Clear
Calculated Concentration:
mg/mL
µM