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Primer T_m Calculator

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Oligo Sequence (5' to 3'):

Monovalent Cation Conc. (e.g., Na⁺, K⁺): i Most standard PCR buffers have a final monovalent cation concentration of 50 mM. If you are unsure, using the default value of 50 mM is a safe assumption.

mM

Results:

Length

GC Content

Basic T_m (Wallace)

Salt-Adjusted T_m

Note: These are estimates. The Basic T_m is for very short oligos (14-20 bases). The Salt-Adjusted T_m is generally more suitable for PCR primers (~20-35 bases). For critical applications, consider software with nearest-neighbor thermodynamic calculations.

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